Hypothesis: When creating a new color you must change the 10th or 11th amino acid. To create black you must add the appropriate amino acids of the particular color.
First red and yellow made orange, then we added Trp, Tyr, and, Phe to the squence to create black. these amino acids that were added disrupted the phobic core.
greenRedwhiteRED Amino Acid
Result: When we saw that the 10th and 11th spot mattered as to what color is produced also led to us to realize that these spots where always in the hydrophobic CORE. We noticed that in the purple structure the tyr and phe were added into the core.
hypothesis : CORRECT
to produce color there must be hydrophobic region that contains Tyrosine, Phenylalanine, or tryptophan. Also one of those three amino acids must be at positions 10 or 11 in the amino acid sequence.
Purple flower
HYPOTHESIS
Our hypothesis is that in order to make a purple flower you must combine the difference in amino acid sequence between blue and red.
EXPERIMENT: We looked at the sequence of amino acid of blue which is Met Ser Asn Arg His Ile Leu Leu Val Tyr Cys Arg Gln. We also looked at the sequence of amino acids of red which is Met Ser Asn Arg His Ile Leu Leu Val Phe Cys Arg Gln. The difference between these two amino acids is the Tyr in blue and Phe in red, they are in the same position in the sequence.In order to obtain a purple flower we added the Tyr and Phe to sequence in different positions.
RESULTS
When we added the phe and tyr to the beginning we got a white color. When we added phe and tyr to the end we got red. When we added phe and tyr to positions 10 and 11 we got purple.
CONCLUSION: In order to make a purple flower you can only add the amino acids phe and tyr to the position 10 and 11 which is the hydrophobic region of the protein. This shows that color and shape is controlled by the sequence of the amino acids in the protein.
Does the shape of the proteins effect the color? Also, does the amino acid sequence of the protein effect the color, if so how?
Hypothesis: The presence of a specific amino acid in the hydrophobic core will impact what color the protein will be. The absence of a specific amino acid will result in colorless. There also must be 6 hydrophilic amino acids and 7 hydrophobic amino acids apart of the core for a color to be present.
Experiment:
Results: When a specific amino acid is apart of the core, it changes the specific color. In this case, Phe creates red, Tyr creates blue, Trp creates yellow, and if these amino acids aren’t present then the result is white. Also, if the number of hydrophobic or hydrophilic amino acids is altered, the color is no longer present.
Conclusion: Our hypothesis was correct and proven.
Hypothesis: To make a protein with color, there should be at least 6 hydrophobic amino acids with one of the following proteins; TYR, PHE, and TRP.
Experiment: We folded a protein that has at least 6 hydrophobic amino acids, and we constructed different proteins with different amino acids besides TYR, PHE, and TRP. We should observe what color the protein makes.
Results: We found that without 7 hydrophobic amino acid in the sequence, the color of the protein will be white. We also found that if the amino acid sequence don’t include TYR, PHE, TRP, then the protein will be white and not show another color.
Conclusion: Our hypothesis is accurate and absolutely correct.
Hypothesis: The amino acids #10 and #11 code for protein color, and manipulating these amino acids can lead to the creation of new colors.
Experiment: We took the proteins coding for Blue and Red and combined them together. They only difference between the structures of the two proteins were at amino acid #10; Blue had Tyr, and Red had Phe.
Results: The addition of the Phe into the Blue amino acid sequence at amino acid #11 (thus increasing the protein chain to 14 amino acids; Cys becomes #12, Arg becomes #13, etc.) caused the resulting color of the new protein to be purple.
Conclusion: Our hypothesis was correct.
We noticed that the true breeding green allele was similar to both the blue and yellow alleles. The only difference was the the 10th protein CYS had been replaced by TRP in the true breeding green. The same was true for when we replaced CYS with PHE in the blue allele, this produced a purple protein. If we were to replace the CYS and the blue allele with PHE from the red allele, it would result in a white protein. This is most likely dependent on the shape because they’re both different.
Hypothesis: Protein color is influenced by the tenth amino acid in the chain.
Experiment: We compared the amino acid sequences of each protein color.
Results: The sequences of the amino acids were identical until the tenth one in the chain.
Conclusion: The hypothesis is valid.
