The Orange protein was created by changing the amino acids at spots 10 and 11 in the amino acid chain to Trp - Phe.
The Purple protein was created by changing the amino acids at spots 10 and 11 in the amino acid chain to Tyr - Phe.
Hypothesis:
Thyrosine 10 resulted in the blue color of the protein.
Experiment:
We modified a blue protein by changing tyrosine 10 to tryptophan.
Result:
As a result we got a yellow protein.
The Conclusion:
Tyrosine at position 10 is responsible for the blue protein.
Hypothesis:
We hypothesized that a specific amino acid was responsible for the color of the protein making it red.
Experiment:
We found that the difference between the yellow and red protein was Phenylalanine, so then we placed the Phenylalanine in a different location on the the reds amino acid sequence.
Result:
The protein was white.
Conclusion:
The hypothesis was incorrect, because the color of the protein depends on the location of certain amino acids.
Hypothesis: Changing amino acid #10 to Tyrosine will change the color to blue .
Experiment: We took a yellow protein and changed amino acid #10 to Tyrosine to see what color would appear.
Result: When you change the amino acid at #10 to Tyrosine , it will change the color of the protein to blue .
Conclusion: Amino acid #10 determines the color of a protein and when that amino acid is changed to Tyrosine, it will change the color of the protein to blue . Our hypothesis was correct .
Hypothesis: We hypothesize that the amino acid Tryptophan, when placed in a hydrophobic “blob”, is responsible for the yellow color of the protein.
Experiment: We took two identical proteins, and switched Trp out for Tyr in one of them.
Result: The protein’s color was changed from yellow to blue.
Conclusion: Trp is directly responsible for the yellow color of the protein.
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