Section 05’s Data Blog

Hypothesis: All of these amino acids are hydrophobic and they all have the benzine ring on their side chains’.

Experiments: We looked back to the Amino Acid chart and compared the side chain’s of Tyr,Phe, and Trp.

Results: When we compared the side chain’s we noticed that all three of the side chains have a benzine ring in their side chains and no other amino acid’s have the benzine ring on their side chains.

Conclusion: According to our data our hypothesis is correct.

Hypothesis: Specific amino Acid don’t matter when replaced with other amino acids of similar charge.

Experiment: We kept the same number of amino acids in the sequence of white, but replaced all the amino acids with ones of similar charge. then replace the second val amino acid with phe in hopes that the color produced would be red.

Result: By changing all the amino acids with ones of similar charge and replacing the second val to phe, when phe produces red, we got red.

Conclusion: it doesn’t matter if you change the amino acid with ones of similar charge. it only matter if you change the specific amino acid in the sequence. This specific amino acid is in the blog: color combo.

Hypothesis: You need a certain number of hydrophobic and hydrophilic amino acids in the protein to be able to make a color.

Experiment: We made proteins with different numbers of  hydrophobic and hydrophilic amino acids.

Results: amino acids with either less than 6 hydrophilic amino acids or less than 7 hydrophobic amino acids the protein won’t have a color.

Conclusion: the hypothesis is correct.

hypothesis: Changing or the amino acid VAL in the color white will produce any other color when adding a specific amino acid in it place.

The Experiment: Change VAL amino acid white to produce a different color. Follow the following chart.

Blue = tyr

Red = phe

Yellow = trp

white = val

Green = tyr & trp

purple = tyr & phe

Black = tyr & trp & phe

orange = trp & phe

The result: By changing one or both Val in white and changing it to one that is above changes the color.

Conclusion: By changing VAL in the sequence of white to one that is with in the chart above does change the color of the flower.

Hypothesis: From the Yellow group , we found out that changing the tenth amino acid in the primary squence of White, we can possibly make purple

Experiment: We place Phe next to the Try in the tenth postion

Result: Protien came out purple

Concluion: Conclusive.

Hypothesis: Tyr and Phe in a protein amino acid sequence codes for purple. But the positioning of the amino acid phe in an amino acid sequence that codes blue determines if the color changes to purple or white.

Experiment: We placed phe near a hydrophobic amino acid. Then we placed phe between two amino acids that are more hydrophilic. We also placed phe at the end of amino acid sequence.

Results: See above pictures- phe next to or between two very hydrophobic amino acids= purple.

phe next to or between hydrophilic amino acids= white.

phe at the terminals of the sequence= white.

Conclusion: The data supports our hypothesis. phe is very hydrophobic and when it interacts with other very hydrophobic amino acids it results to color change (purple).

Hypothesis: If we substitute a hydrophilic amino acid with a hydrophobic amino acid it will change color.

Experiment: We started out with 13 amino acids, then we replaced 2 hydrophilic amino acid (Arg Gln) with 2 hydrophobic amino acids (Ile Ile). Then in another sequence we went back and replaced the hydrophobic amino acids (Ile Ile) with two new hydrophilic amino acids (Asn Asn).

Results: When we replaced the hydrophilic amino acid with a hydrophobic amino acid we lost the original green color and got the color white. Then when we put back two new hydrophilic amino acids (Asn Asn) we noticed that the original color came back.

Conclusion: Our data supports our hypothesis. If we substitute a hydrophilic amino acid with a hydrophobic amino acids it will change color.

Hypothesis - Adding more hydrophobic atoms to a blue protein will make it change color.

Experiment - We added Ile Leu Val Val for the upper level. Next we added Leu Leu Leu Ile Val for the lower level.

Result - It stayed the same color, blue.

Conclusion - inconclusive

hypothesis: placing a hydrophilic on the outsides of the tyr and phe will keep it from being purple

Experiment: we placed two hyrdophilic amino acids on the outsides of the tyr and phe

Result: it changed the color

conclusion: agrees with hypothesis the protien was white and not purple