Section 08’s Data Blog

Hypothesis: In the amino acid sequence color change depends on the amino acids in location #10 and #11.  The purple flower has tyr at #10 and phe at #11.  If these are swtiched the color is changed.

Experiment: We switched tyr from #10 to #11 and phe from #11 to #10.

Results:

Tyr in location #10 switched with phe in location #11

Original purple with tyr in location #10 and phe in location#11

Conclusion: The switch caused the color to change from purple to white because of the hydrogen bond formed between tyr and gln.  Our hypothesis was accurate.

Hypothesis: By combining the 10th amino acids in both protein’s sequences, the resulting protein will be colored purple.

Experiment: We compared the sequences of the red and blue proteins, and noticed the only difference was in the 10th amino acid. We decided to put in the two 10th amino acids in a new protein.

Result: Adding Tyr from position 10 of the blue protein, and Phe from position 10 of the red protein to the new protein resulted in purple coloration.

Conclusion: Our hypothesis was correct, by combining the two amino acids at position ten of both the red and blue proteins, while keeping the sequence of the rest of the proteins the same, results in purple coloration.

Hypothesis: Changing 10th amino acid from Valine to Phenylalanine will change the color of the protein from white to red.

Experiment: We changed the amino acid at position 10 from Valine to Phenylalanine.

Result: The color changed from white to red.

Conclusion: Changing the sequence of amino acids will alter the color of the protein.

The active proteins are in positions 10 and 11 in the amino acid sequence; cysteine is merely a place-holder (it appears as white, and the recessiveness means that it is inactive).

This means that all the amino acids that are not cysteine in position 10 and 11 are tied to a specific color. Tyr for blue, Trp for yellow, phe for red. By mixing the proteins in position 10 and 11, you can produce the mixed colors. Trp and Tyr for green, phe and tyr for purple.

Valine also acts as place-holder, producing white.

Another example: phe and trp in position 10 and 11.

Hypothesis:We can create a protein that is purpleand has 13 spaces instead of 14 and that if we swiched the amino acids in spaces 10 and 11 we would produce a purple protein.

Results:

We Tested the purple protein and found that we can create a protein with 13 spaces in the following sequence.

Met Ser Asn Arg His Ile Leu Leu Val Tyr Phe Arg Gln

but when we switched 10 and 11 we got white proten.

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