Section 16’s Data Blog

Hypothesis:

ong thin proteins without a hydrophobic blob containing tyrosine, tryptophan, or phenyalalnine are white.

Experiment:

we designed a long thin protein looked at the result and then added a hydrophobic blob of amino acids and checked for a color change.

Result

The long thin protein was white and adding a hydrophobic blob of amino acids containing tryrosine, tryptophan, or phenyalalnine changed the color.

White protein:

Colored protein:

Hypothesis: The features of a protein that make it colored are the types of amino acids at the 10 and 11 position.

Experiment: We rearranged the amino acid in position 10 to see if the color would change.

Results: The color changed.

Conclusion: Our hypothesis was correct

both blue and red proteins have the same shape regardless of the fact that in position 10 of blue has Tyr and in red it Phe. Tyr is polar and Phe is non-polar yet the shape of the protein is preserved because their side chains both contain a large carbon ring and final size is similar.

Hypothesis: If we switch amino acid #10 to val, it will create a mutation and become the color white

Experiment: we took the amino acid sequence of yellow and switched a.a. #10 from tyr to val

Result: mutation into the color white

Pics:

a) Our hypothesis is thyrosine makes a protein blue.

b) We substituted thyrosine with threonine.

c) It resulted in the protein changed its structure and became white.

d) Our initial hypethesis that thyrosine would have to be next to hydrophobic blobs to be blue.

Changing the 10th and 11th amino acids to Tyr and Phe makes purple. Cys is recessive to all.

Hypothesis: Combinding the change in color sequence from Blue and Red give you purple. Blue’s color comes from position 11 and red comes from position 10. Combing 10 and 11 with the regular sequence gives you a purple protein.

The 10th and 11th amino acid produces the color of the protein. cys has no effect, tyr adds blue, trp adds yellow, phe adds red, val adds white.

Hypothesis: color is determined by shape of the protein; changing hydrophobic amino acid at #11 in sequence to a hydrophillic amino acid leads to a white color

Experiment: switch #11 amino acids with a hydrophillic molecule and record the resulting colors

Results: white for each sequence in which #11 was changed to a hydrophillic molecule

Conclusion: our hypothesis is correct!

Check out our pic!!!

 

 

 

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